Pefabloc® SC

Pefabloc® SC an irreversible protease inhibitor with broad specificity for serine proteases.

With its superior solubility, stability, inhibitory activity and low toxicity, Pefabloc® SC is suitable for biopharmaceutical downstream purification. It belongs to the family of sulfonyl fluorides which irreversibly block serine proteases. Pefabloc® SC is a potent serine threonine phosphatase inhibitor.

APPLICATION:
Pefabloc® SC is used to inhibit the detrimental effects of proteases during preparative protein purification. Due to its low toxicity toward eukaryotic cells, it may be applied in the production of recombinant proteins, during fermentation of transformed cells, where proteolytic digestion may decrease the yield of the desired product. It can be used advantageously to completely inactivate protease K during the preparation of chromosomal DNA in agarose plugs.

In contrast to PMSF, Pefabloc® SC is an excellent blocker of thrombin activity in serum or plasma. In these biological fluids, PMSF interacts in a reversible manner with albumin, which reduces its free concentration and leads to a delay in thrombin inactivation. Pefabloc® SC, however, does not react with serum albumin, and exhibits a threefold higher capacity to inactivate thrombin under similar conditions.

Use Pefabloc® SC to inactivate protease K, for example, during pulsed-field gel electrophoresis (PFGE). With this technique, isolating the genomic DNA requires protease K to degrade cellular components, and this highly resilient protease is difficult to inactivate. Pefabloc® SC inhibits protease K, and protects the stability of restriction enzymes used for further DNA analysis

ADVANTAGES:
Because it is water soluble, Pefabloc® SC is easy to use and can be directly added to aqueous buffers. And because it is non-toxic, it can be readily employed where the use of hazardous compounds represents a risk. Finally, because of its high stability, consistent protease inhibition can be achieved even at pH levels above 7.0 and temperatures above +4°C.