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Pefakit® TAFI Pefakit® TAFI is a plasma based chromogenic assay for determination of Thrombin Activatable Fibrinolysis Inhibitor (TAFI) enzyme activity. The synthetic substrate is a substituted peptide mimetic consisting of an amino-protected L-lysine connected with an L-arginine of which the α-position of the side chain is a sulphur atom. It is degraded selectively and irreversibly by TAFIa producing a thiol derivative. This thiol reacts chemically with the colourless Ellman’s reagent (5,5’-Dithio-bis-(2-nitrobenzoic acid), DTNB) splitting off the yellow coloured 5-mercapto-2-nitro-benzoic acid. The extinction measurable at the wavelength of 405 nm at the end of the enzymatic reaction is directly proportional to the concentration of TAFI activated by thrombin/thrombomodulin. Features · Funcional assay based on direct substrate cleavage · Specific for TAFI · Applicable for MTP and coagulation instruments · Easy to use: short turn around time Pefakit® TAFI Calibrator and Controls Calibrator and control plasmas are used for calibration and control of the plasma based chromogenic assay for determination of Thrombin Activatable Fibrinolysis Inhibitor (TAFI) enzyme activity with Pefakit® TAFI (REF 800186). Features · Easy to use · Stable reagents |